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KMID : 0620920090410120880
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Experimental & Molecular Medicine
2009 Volume.41 No. 12 p.880 ~ p.895
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Activation of PPARγ induces profound multilocularization of adipocytes in adult mouse white adipose tissues
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Koh Young-Jun
Park Jin-Woo
Park Byung-Hyun
Lee In-Kyu
Park Ji-Hyun
Han Jin-Ah
Koh Gou-Young
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Abstract
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We sought to determine the effects of activation of peroxisome proliferator-activated receptor-γ (PPAR-γ) on multilocularization of adipocytes in adult white adipose tissue (WAT). Male C57BL/6 normal, db/db, and ob/ob mice were treated with agonists of PPAR-γ, PPAR-α , or β3-adrenoceptor for 3 weeks. To distinguish multilocular adipocytes from unilocular adipocytes, wholemounted adipose tissues were co-immunostained for perilipin and collagen IV. PPAR-γ activation with rosiglitazone or pioglitazone induced a profound change of unilocular adipocytes into smaller, multilocular adipocytes in adult WAT in a time-dependent, dose-dependent, and reversible manner. PPAR-α activation with fenofibrate did not affect the number of locules or remodeling. db/db and ob/ob obese mice exhibited less multilocularization in response to PPAR-γ activation compared to normal mice. Nevertheless, all adipocytes activated by PPAR-γ contained a single nucleus regardless of locule number. Multilocular adipocytes induced by PPAR-γ activation contained substantially increased mitochondrial content and enhanced expression of uncoupling protein-1, PPAR-γ coactivator- 1-α , and perilipin. Taken together, PPAR-γ activation induces profound multilocularization and enhanced mitochondrial biogenesis in the adipocytes of adult WAT. These changes may affect the overall function of WAT.
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KEYWORD
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mitochondria, mitochondrial uncoupling protein, pioglitazone, receptors, adrenergic, β-3, rosiglitazone
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